Multiplexed one-step RT-PCR VP7 and VP4 genotyping assays for rotaviruses using updated primers

One-Step Reverse Transcription PCR (RT-PCR). Fatemeh Mehrpooyan*

Designing and Validation of One-Step T-ARMS-PCR for Genotyping the eNOS rs1799983 SNP

Background: The transversion of G to T (G894T) in human endothelial nitric oxide synthase (eNOS) gene has profound effects such as male infertility, recurrent miscarriage, multiple sclerosis and cardiovascular diseases.Objectives: Development of a new Multiplex Tetra-Primer Amplifi cation Refractory Mutation System - Polymerase Chain Reaction (T-ARMS-PCR) for detection of...

One-step real-time RT-PCR assays for serotyping dengue virus in clinical samples

BACKGROUND Dengue is one of the leading causes of morbidity in tropical and subtropical regions and infection with any of the four dengue virus serotypes (DENV1-4) result in a wide range of clinical manifestations. Given the geographic expansion of DENV1-4, assays for serotyping are needed to be able to perform surveillance and epidemiological studies. In this study, we describe the design and ...

Parallel picoliter rt-PCR assays using microfluidics.

The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel singl...

One-step RT-PCR for screening microdissected tissue samples.

The technology of laser-capture microdissection (LCM) enables the easy extraction of single cells or defined groups of cells from a tissue section (1,2). This technique uses a laser pulse to activate a plastic polymer, which binds to and captures cells from a frozen or paraffin-embedded tissue section. Captured cells present on this solid support can then be used for nucleic acid and/or protein...